The main main push process of megachromatography is the most advanced sequential simulated moving bed chromatographic separation process in the world. This process has the characteristics of low investment, high separation efficiency, simple control and convenient operation. The spectroscopy is divided into four-column mode, six-column mode, and six-column four-column mixing mode according to the number of columns. According to the amount of the separated components, the two-component mode, the three-component mode, and the two-component separation mode of the two components are divided.
1, according to the number of columns
1.1 four-column mode
There are only four columns in the four-column mode, four circulating pumps, and the control valve is also reduced by one-third. It is the simplest structure, the simplest control mode, and the most economical mode. It is suitable for the separation of materials that are less difficult to separate or that require less purity. Like F55 fructose plant, F42 fructose pulp can be separated by chromatography. The purity of sugar is about 90%, and 85% can be. However, it is not suitable for materials with high separation difficulty and high purity requirements, because the total length of the column in the four-column mode is short, the separation stroke is short, and it is difficult to separate the components which are difficult to separate.
1.2 six-column mode
There are six columns in the six-column mode, and six circulating pumps. Because of the long total length of the column and the long separation length, the separated components can be sufficiently separated, so this mode is suitable for separation difficult or separation purity requirements. Higher separation of materials. This mode is used in most chromatographic separation projects.
1.3 six-column four-column mixing mode
This mode also has six chromatograms, six circulating pumps, but at the No. 4 chromatographic outlet there is a circulating line interface and control valve that loops back to the No. 1 column, and the No. 1 to No. 4 chromatographs are connected in series to form a 4-column mode. In this mode, the control computer has six sets of six-column and four-column control software, corresponding to the six-column and four-column modes in actual use. In the peak season, the 6-column mode is used to increase production capacity. In the off-season, the 4-column mode is used to save energy and reduce consumption.
2. Classified by the number of separated components
2.1 two-component separation mode
In a homogeneous mixture, no matter how many components it contains, it can be divided into two groups according to the peak speed in the chromatographic resin: fast component group and slow component group. The chromatographic separation mode in which the homogeneous mixture is chromatographed into two components is the two-component separation mode. The two-component separation mode is relatively simple, and is suitable for purification and separation of materials with the fastest or slowest peaks of the target components. If the fructose in the glucose syrup has the slowest peak, the oligosaccharides in the various oligosaccharide liquids have the fastest peaks, which are suitable for the separation of the two components. The two-component separation mode currently used most in the starch sugar industry.
2.2 Three-component separation mode
2.2.1 Separation mode
In a homogeneous mixture, no matter how many components it contains, it can be divided into fast component C, intermediate component B, and slow component A according to the peak of its peak in the chromatographic resin. The target component of the three-component chromatographic separation is intermediate component B. The three-component separation process is varied, but the best results at present are concentrated feed processes. The characteristic of this process is that, in a large cycle, the raw materials are completely discharged from the intermediate component region, and then the component C and the slow component A are separated in multiple times, and the intermediate component B is temporarily not present, and the intermediate component is to be After B is sufficiently purified, the intermediate component B is discharged in one shot.
This process is completed in a few steps and is now illustrated in only three steps. In each step, the feed position is followed by the B component. Step 1: Enter the raw material A+B+C. See Figure 1: At this time, only the raw material ABC is fed, and no material is discharged.
Figure 1 Schematic diagram of step 1
Step 2: A, C. During the separation process, the fast component C and the slow component A are moved forward and backward, respectively, and are discharged before and after, while the intermediate component B is purified and accumulated at the intermediate position without discharging. This step needs to be repeated several times to allow the three components to be sufficiently separated. See Figure 2:
Figure 2 Schematic diagram of step 2
Step 3: Extract the B component in the middle, and A and C do not discharge. At this time, the three components A, B, and C are sufficiently separated, and the purified and enriched component B is taken out. See Figure 3.
Figure 3 Schematic diagram of step 3
Through these three steps, the three components can be well separated. This mode is characterized by one-time feeding, multiple times A and C, and the B is fully extracted after being fully purified. Therefore, the intermediate component B of this mode is of high purity. In this mode, the three components are separated from each other in a chromatographic system, and the resin space occupied is large. Therefore, the resin treatment amount is low and the water consumption is large. Purification of the mannitol mother liquor by this mode, the separation and purification of trehalose mother liquor is very effective. This separation mode is the latest technology for the successful development of our company. As the three-component separation becomes more and more concerned, this high-efficiency three-component chromatographic separation mode should have broad market prospects.
2.2.2 Scope of application
The application range of the three-component separation of the simulated moving bed is also wide, and many applications are in the pharmaceutical field. The application in the range of starch sugar is now listed below:
Separation component application example product
Glucose / xylose / arabinose xylose mother liquor xylose arabinose
Oligosaccharide / glucose / mannose mannose production mannose glucose
Heterool / mannitol / sorbitol mannitol mother liquor mannitol sorbitol
Oligosaccharide / glucose / fructose fructose oligosaccharide glucose fructose
Oligomer / maltitol / sorbitol maltitol purification maltitol oligosaccharide / disaccharide / monosaccharide
Inorganic and non-sugar / sucrose / monosaccharide sucrose honey sucrose
Inorganic salts and non-sugar / sucrose / betaine beet molasses sucrose betaine
2.3 Two-component separation mode
The main separation form of this mode is completely a two-component separation mode, except that the discharge time of one of the two components is divided into two sections, the first-out section and the intermediate component are relatively small, thereby relatively increasing The purity of the intermediate component. Because the discharge composition of the chromatographic system is constantly changing with the discharge time. For the two-component separation mode, the concentration of fast component B gradually increases with the discharge time, and its purity gradually decreases with the discharge time, because the inclusion amount of A in component B gradually increases; The concentration gradually decreases with the discharge time, but its purity gradually increases with the discharge time, because the inclusion amount of B in component A is gradually reduced. For example, we can change the pipe of B to 2, and divide the entire B process into two segments B1 and B2 by time or volume. B1 and B2 are collected from two pipes in different containers. If the volumes of B1 and B2 are properly controlled, then B1 contains more of the fastest components and the intermediate component of B2 is of higher purity.
In summary, the essence of this mode is the two-component separation mode, which only cuts the output of B or A into two segments according to different discharge times of the chromatogram and different properties of the discharge components, thereby relatively improving the middle. Target product purity. The characteristic of this mode is that the control is simple, the throughput and the water consumption are exactly the same as the pure two-component separation, that is, the processing volume is large and the water consumption is low. However, this mode has a limited degree of purification of the intermediate component because it does not leave sufficient time for the third component to pass through the intermediate component, and the intermediate component is constantly entangled with the third component.
Advantages of megachromatography:
1. Advanced Process The sequential intermittent operation process (SSMB) greatly improves system efficiency, and system performance and economy have greater advantages than traditional processes (SMB).
2. According to the process requirements and column specifications, the independent and scientific design of the distributor mode, to minimize the back-mixing of the liquid in the system to improve the separation efficiency of the system.
3. Practical training of talents and professionalization of talents
Zhaoguang Chromatography Co., Ltd. adheres to on-demand training in personnel training, and implements professional equipment in personnel training to ensure professional technical innovation ability and full-time professional quality;
4. Full technical tracking, all-weather technical service
Megachrome has implemented a full-service technology tracking service. Regular consultation on customer operation, on-site close to service to solve practical problems, and achieve high efficiency in appeal response;
5. Advanced design concept, stable system operation
The overall design adheres to the principle of combining practicality with practice. The system design coordinating various factors, making the equipment performance efficient, stable, and long service life; the control parameter design is user-friendly, and the control is convenient and the adjustment is fast;
6. Many engineering projects and rich engineering experience
Chromatography projects are spread all over the country, and have accumulated a large number of industrial practical experience, providing support for the continuous innovation of chromatographic technology systems.
7. Low investment and high quality, cost-effective
On the same scale, the same configuration of the chromatographic system device, in the case of ensuring that the purity, yield, water consumption and other technical indicators are not lower than the foreign similar equipment, the price of the mega-chromatography system device is lower than the foreign ones by more than 25%.
8. Strong technical foundation and rich technical reserves
The company has a complete research and development platform and sufficient processing service capabilities, can provide turnkey projects and a series of technical services, and can carry out technical cooperation with customers in the whole industry chain. With years of research and development and continuous innovation, the omni-chrome chromatography technology is comprehensive and complete, ensuring the chromatographic technology and equipment requirements of various customers.
Mature chromatographic technology reserve
1. Purification and separation technology of erythritol mother liquor;
2. Separation and purification technology of ribose and ribose mother liquor;
3. Mannose separation and purification technology;
4. Three-component maltitol separation and purification technology;
5. Organic acid (citric acid, lactic acid) separation and purification technology;
6. Amino acid (threonine, lysine, etc.) purification technology;
7. Pharmaceutical chiral separation technology;
In addition, we can provide customers with services such as separation media evaluation and technical feasibility analysis;